Carcinoma differentiation dissertation embryonic epigenetic p19 study
Genome-wide demethylation during neural differentiation of
In this study, we investigated the possible role of Sox6 in neuronal cell differentiation of murine P19 embryonic carcinoma (EC) cells . For the induction of neuronal differentiation, P19 cells were allowed to aggregate for 4 days in the presence of retinoic acid (RA) and were replated for 5–7 days without RA.
Chemical Induction of Cardiac Differentiation in P19
This study presents the first evidence for epigenetic changes occurred on a specific KOR promoter triggered by NGF in cells undergoing neuronal differentiation. This epigenetic change is mediated by recruited AP2β to this promoter and involves the PI3K system. KW - AP2. KW - NGF. KW - P19 neuronal differentiation. KW - epigenetic
Differential effects of AdOx on gene expression in P19
B. D. Smolich and J. Papkoff, “Regulated expression of Wnt family members during neuroectodermal differentiation of P19 embryonal carcinoma cells: overexpression of Wnt-1 perturbs normal differentiation-specific properties,” Developmental Biology, vol. 166, no. 1, pp. 300–310, 1994. View at: Publisher Site | Google Scholar
Epigenetic Activation of μ-Opioid Receptor Gene via
Resende R.R., Majumder P., Gomes K.N., Britto L.R., Ulrich H. P19 embryonal carcinoma cells as in vitro model for studying purinergic receptor expression and modulation of N-methyl-D-aspartate-glutamate and acetylcholine receptors during neuronal differentiation. Neuroscience. 2007; 146:1169–1181. [Google Scholar]
An Efficient Protocol for Embryonic Carcinoma Cells P19
Feb 08, 2013 · P19 embryonal carcinoma (EC) cells are pluripotent stem cells and have numerous morphological and biochemical properties in common with embryonic stem (ES) cells. However, P19 cells differentiate very ineffectively as embryoid bodies (EBs) without the specific chemical inducers whereas ES cells exhibit spontaneous differentiation to the three germ layers.
Histone Deacetylase Inhibitors in Cell Pluripotency
For differentiation we used a novel co-culture system with the human NTERA-2, a committed neuronal precursor line and P19, a murine embryonic carcinoma line . Previously, in vitro differentiation to lens cells was achieved by co-culture [ 21 ], but also by supplementation of the culture media with defined growth factors, such as Noggin, FGF2
Human Embryonal Carcinoma Stem Cells: Models of Embryonic
Apr 06, 2012 · The murine P19 embryonic carcinoma cell (ECC) model was used to recapitulate developmental processes of cardiomyogenesis in vitro . ECCs have a high capacity to form cardiomyocytes in vitro and exhibit during differentiation the biochemical and physiological properties of their embryonic equivalents in vivo during cardiomyogenesis , . For the first time …
Evidence of endogenous mu opioid receptor regulation by
In this study, using an embryonal carcinoma (EC) P19 stem cell neuronal differentiation system, we demonstrate that the expression of CoAA dramatically switches to its alternative splicing variant CoAM in cells destined to form the cavity of embryoid bodies (EBs).
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Mechanisms Regulating Stemness and Differentiation in
Sep 29, 2004 · Embryonal carcinoma (EC) cells are pluripotent stem cells derived from teratocarcinomas and are considered the malignant counterparts of human embryonic stem (ES) cells. Several of the existing human EC stem cell lines provide robust and simple culture systems to study certain aspects of cellular differentiation in a manner pertinent to human
Depletion of Embryonic Stem Cell Signature by Histone
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Dural effects of oxidative stress on cardiomyogenesis via
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Abstract Aim: In current study capability of differentiation of embryonic carcinoma cells P19 to cardiomyocyte through inducing effects of DMSO and 5-Azacytidin individually or in combinative form
Nuclear cloning of embryonal carcinoma cells | PNAS
Epigenetic regulation including DNA methylation plays an important role in several differentiation processes. We profiled global DNA methylation in the neural differentiation of P19 embryonic
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Interestingly, embryonic carcinoma cells (NCCIT, NTERA2, and P19) exhibit a higher sensitivity to apicidin in down-regulation of Nanog compared with embryonic stem cells. Furthermore, the down-regulation of Nanog expression by apicidin is mediated by a coordinated change in recruitment of epigenetic modulators and transcription factors to the
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Stage-specific embryonic antigen 1 (SSEA1) plays a role in cell adhesion and migration in the pre-implantation embryo and is widely used as a pluripotency marker in mouse ESCs, and is also expressed in embryonal carcinoma cells, including P19 cells [].Immunofluorescence microscopy reveals a reduction in SSEA1 in both Hmgn1 and Hmgn2-knockout cells, and quantification by FACS indicates a
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Embryonal carcinoma (EC) cells have served as a model to study the relationship between cancer and cellular differentiation given their potential to produce tumors and, to varying degrees, participate in embryonic development. Here, nuclear transplantation was used to assess the extent to which the tumorigenic and developmental potential of EC cells is governed by epigenetic as opposed to
Switched alternative splicing of oncogene CoAA during
Stable epigenetic control of gene function is a crucial part of normal development of most complex organisms, especially higher plants and mammals. We used the P19 embryonal carcinoma in vitro model of neurogenesis to study various aspects of epigenetic regulation of gene expression. ^ Most cases of Angelman syndrome result from inactivation or deletion of ubiquitin ligase 3A (UBE3A), a …
Stepwise renal lineage differentiation of mouse embryonic
enhanced Ezh2 expression. In the third study, we examined arsenic’s effects earlier in development, using mouse P19 embryonic carcinoma cells as our model. These results indicate that arsenic inhibits myogenesis and neurogenesis due to the reduction of essential myogenic and neurogenic transcription factors, such as Pax3, Myf5,
Epigenetic studies of P19 embryonic carcinoma differentiation
Neuronal Differentiation of P19 Cells. P19 embryonic carci-noma cells were cultured and differentiated into neuronal cells as previously described (Hwang et al., 2007; Wagley et al., 2013) with minor modifications. Briefly, for induction of differentiation, 5 106 cells were cultured in differentiation medium [a-MEM (Life Techno-
Foxm1 transcription factor is required for maintenance of
As P19 EC cells are frequently used as an in vitro model system to study the developmental milestone events, such as the differentiation of neurons , P19 EC cells were treated with reagents that induce epigenetic modifications to determine whether Nestin undergoes epigenetic regulation. Cells were treated with either 5‐aza‐dC, an inhibitor
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Mouse embryonic stem (ES) cells are pluripotent, as they have the ability to differentiate into the various cell types of a vertebrate embryo. Pluripotency is a property of the inner cell mass (ICM), from which mouse ES cells are derived, and of the epiblast of the blastocyst. Recent extensive molecular studies of mouse ES cells have revealed the unique molecular mechanisms that govern
How is pluripotency determined and maintained? | Development
Sep 28, 2004 · Embryonal carcinoma (EC) cells have served as a model to study the relationship between cancer and cellular differentiation given their potential to produce tumors and, to varying degrees, participate in embryonic development. Here, nuclear transplantation was used to assess the extent to which the tumorigenic and developmental potential of EC cells is governed by epigenetic as …
A Quantitative Proteomic Analysis of Mitochondrial
Histone deacetylase inhibitors (HDACi) are small molecules that have important and pleiotropic effects on cell homeostasis. Under distinct developmental conditions, they can promote either self-renewal or differentiation of embryonic stem cells. In addition, they can promote directed differentiation of embryonic and tissue-specific stem cells along the neuronal, cardiomyocytic, and hepatic
Study identifies interaction that promotes cancerous state
Abstract. P19 embryonal carcinoma cells (EC-cells) provide a simple and robust culture system for studying neural development. Most protocols developed so far for directing neural differentiation of P19 cells depend on the use of culture medium supplemented with retinoic acid (RA) and serum, which has an undefined composition.
ARSENIC REPRESSES MYOGENESIS AND NEUROGENESIS
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or alter the expression of microRNAs that are important in stem cell differentiation. We used P19 murine embryonic stem cells, which were exposed to 0, 0.25, or 0.5 µM arsenite for up to 9 days, to analyze the expression of developmental-related cell signaling pathways and microRNAs by microarray and quantitative PCR.
Sox6 overexpression causes cellular aggregation and the
Jul 13, 2006 · All‐trans RA is a potent inducer of differentiation of undifferentiated, highly proliferative cells, such as ES cells or embryonal carcinoma (EC) cells. In vitro , RA‐induced differentiation of these cells gives rise to specific cell types in a time‐ and concentration‐dependent manner ( Strickland & …